The study aimed to assess the impact of various drugs of abuse (DOA) on urine parameters, specifically potential hydrogen (pH), specific gravity (SG), and creatinine (CART) levels within a dataset of 401 human urine samples. The Enzyme-Linked Immuno-Assay (ELISA) method was employed to measure these parameters, and subsequent statistical analysis using Systat statistical software was conducted following data cleaning. When comparing the pH, SG, and CART values of samples with and without DOA presence, no statistically significant differences were observed. Initially suggesting that the presence of DOA does not substantially affect these urine parameters. However, these preliminary findings may imply that pH, SG, and CART levels in urine samples are not directly influenced by the presence of DOA; instead, other factors such as diet, hydration, and individual physiology may play a more significant role. The limited dataset size may contribute to this outcome, emphasizing the need for a more comprehensive investigation with a larger and more diverse dataset. In future, further analysis will be carried out with a control group with carefully collected, unadulterated urine samples from healthy individuals with larger dataset. It will allow the meaningful comparisons and a deeper understanding of the relationship between DOA and urine parameters. Furthermore, various alternatives parameters will be explored to improve drug abuse detection and prevention strategies.