It is now well understood that the indiscriminate use of agrochemicals under conventional agriculture not only causes severe health hazards for human beings but also has numerous other side effects on the environment including destruction of the biodiversity. Endosulfan is a pesticide belonging to the organochlorine group of pesticides, under the Cyclodiene subgroup. It is used in vegetables, fruits and timber crops. Present study illustrates effect of endosulfan on histopathology of Kidney of mice. Mice were divided into group of 10 for each control and treated group. Treated group receives 2 mg/kg b.w/day endosulfan for three and five weeks. It is observed that in control group cortex and medulla region of kidney were well organized. Glomerulus, bowmens capsule, PCT and DCT were normal. While in endosulfan 2mg/kg b.w/ day for three weeks administered group clustered cells of bowmens capsule and glomerulus with prominent vacuolization in cortex region of kidney were observed. Degenerated medulla region were also observed. Endosulfan 2mg/kg b.w/ day for five weeks administered group show Clustered nuclei in Glomerulus, rudimentary bowmens capsule were also observed. Vacuolization were observed in glomerulus scattered cytoplasm were also observed on cortex region of kidney cortex region of kidney. Distal convoluted tubule and collecting duct were observed with degenerated cytoplasm which causes improper absorption of water causing polyuria condition. It finally indicates that histopathological alteration in kidney with degenerated Glomerulus, proximal convoluted tubule and collecting duct leading to renal disfunction in mice due to improper filteration, absorption and re-absorption process.