International Journal of

Toxicological and Pharmacological Research

e-ISSN: 0975 5160

p-ISSN: 2820-2651

Peer Review Journal

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1.Ease of Dual Priming Oligonucleotide Technology for the Genotyping of Herpes Simplex Virus.
Narotam Sharma, Satish.C.Nautiyal, MD. Zohaib Ahmed, Neha Saini, Renu Dhar, Harshita Verma, Bhawana Bhatt, Yogyata Jadli, R.K. Singh
Herpes simplex virus (HSV) encephalitis is a serious infection but diagnosis previously required brain biopsy in certain cases due to the low sensitivity of cerebrospinal fluid (CSF) culture and serology. PCR allows the detection of HSV DNA from CSF with 95% sensitivity thus avoiding invasive brain biopsy. HSV type 1and type 2 is highly contagious, and can infect skin, mouth and genital organs, both in male and females. Present study, includes the genotyping of HSV type 1 and 2 with the utilization of DPO (dual priming oligonucleotides) primers by conventional PCR. DPO PCR detected 2 out of 8 specimens with HSV infection. Though, study needs the trials of the technology on more number of specimens which can be useful as compared to serological or culture techniques for Herpes Simplex Virus.

2. Effect of Phytochemical Constituents of Ricinus Communis, Pterocarpus Santalinus, Terminalia Belerica on Antibacterial, Antifungal and Cytotoxic Activity
Monisha K, Vandita P, Nirali Amin, Khyati P
Three medicinal plants, Ricinus communis, Pterocarpus santalinus, Terminalia belerica were studied for their phytochemical constituents showing antibacterial activity, antifungal activity and cytotoxicity. In phytochemical analysis, results showed the presence of tannins, alkaloids, cardiac glycosides, terpenoids, flavonoids and steroids in the all three plants. Antibacterial activity was tested against human pathogenic micro-organisms like B. subtilis, S. aureus, S. abony, E.coli, P. aeruginosa by agar well diffusion method. Antifungal activity was tested against certain pathogenic fungi A. niger, C. albicans, Rhizopus, Lasiodiploidia theobromeae and A. solani by pour plate method.  The cytotoxic effect of selected plants were tested against HEK293T (Human embryonic kidney cell line) and c2c12 (Mouse, Muscle cell line) by MTT assay. Results showed that selected plants contain important phytochemicals which can be used to investigate its potential use for developing new drugs.

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