Herpes simplex virus (HSV) encephalitis is a serious infection but diagnosis previously required brain biopsy in certain cases due to the low sensitivity of cerebrospinal fluid (CSF) culture and serology. PCR allows the detection of HSV DNA from CSF with 95% sensitivity thus avoiding invasive brain biopsy. HSV type 1and type 2 is highly contagious, and can infect skin, mouth and genital organs, both in male and females. Present study, includes the genotyping of HSV type 1 and 2 with the utilization of DPO (dual priming oligonucleotides) primers by conventional PCR. DPO PCR detected 2 out of 8 specimens with HSV infection. Though, study needs the trials of the technology on more number of specimens which can be useful as compared to serological or culture techniques for Herpes Simplex Virus.

Three medicinal plants, Ricinus communis, Pterocarpus santalinus, Terminalia belerica were studied for their phytochemical constituents showing antibacterial activity, antifungal activity and cytotoxicity. In phytochemical analysis, results showed the presence of tannins, alkaloids, cardiac glycosides, terpenoids, flavonoids and steroids in the all three plants. Antibacterial activity was tested against human pathogenic micro-organisms like B. subtilis, S. aureus, S. abony, E.coli, P. aeruginosa by agar well diffusion method. Antifungal activity was tested against certain pathogenic fungi A. niger, C. albicans, Rhizopus, Lasiodiploidia theobromeae and A. solani by pour plate method.  The cytotoxic effect of selected plants were tested against HEK293T (Human embryonic kidney cell line) and c2c12 (Mouse, Muscle cell line) by MTT assay. Results showed that selected plants contain important phytochemicals which can be used to investigate its potential use for developing new drugs.