A rapid, simple method is presented for the identification and determination of pregabalin (PREG) in human urine by gas chromatography mass spectrometry (GC/MS) Solid phase extraction was performed using Strata TM-X-C33 µm polymeric strong cation columns (100 mg/3 mL tubes) to collect the extracts. The eluted extract was then derivatized directly in the column using N-methyl-N-(trimethylsilyl) trifluoroacetamide containing 1% trimethylchlorosilane (MSTFA). This method is specific for identifying PREG in urine, even when more than 10 different drugs of abuse are present. It easily detects both PREG-H2O MSTFA and PREG 2MSTFA, which have a relative retention time (RRt) of 0.9. At 1000 ng/ml, absolute recovery is 94.2%. Within-day precision (CV%, n=5) is 8.1% for PREG 250 and 0.3% for 1000 ng/ml. The method demonstrates linearity over 250-1000 ng/mL, with a limit of detection (LOD) of 250 ng/mL and a limit of quantitation (LOQ) of 500 ng/mL. These results show the method is highly suitable for routine identification of the drug in toxicology trace analysis.